HCBI: Zeiss ELYRA
HCBI: Zeiss ELYRA

Widefield, laser widefield, TIRF, spectral confocal imaging, and super-resolution? The ELYRA can do it all! Using structured illumination, this system can improve resolution in X, Y, and Z two-fold – with no special sample prep needed. If that isn’t sufficient for your experiment, photomanipulation super-resolution techniques like PALM or dSTORM can improve your resolution by as much as an order of magnitude! Clicking a button in the ZEISS software easily switches between imaging modes, allowing you to image the same sample by a variety of techniques.

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HCBI: Bruker True Live 3D
HCBI: Bruker True Live 3D

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HCBI: Zeiss 2-Photon
HCBI: Zeiss 2-Photon

Two-photon illumination has always appealed to biologists for imaging deep into living tissue. But did you ever imagine probing more than 5mm into your sample? Using the 780 NLO with our specialized SCAlE objective, you can visualize deep into clarified tissue with incredible resolution. Even without clarifying your samples, this microscope excels at penetrating deep into live or fixed specimens, and creating beautiful 3D datasets.

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HCBI: AxioZoom V16
HCBI: AxioZoom V16

Combining a 16x zoom with high numerical aperture objectives, the AxioZoom.V16 offers superior brightness in large object fields. This highly versatile microscope can image samples illuminated by transmitted, reflected, polarized, and fluorescent light sources, and is equipped with high resolution color and monochrome cameras. The AxioZoom.V16 also boasts an Apotome, an optical sectioning device, capable of creating large field 3D images.

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HCBI: LSM 700 Inverted Confocal
HCBI: LSM 700 Inverted Confocal

So you have a four-color sample that requires 3D imaging over large tiled areas? Then the LSM 700 is the microscope for you. This incredibly user-friendly point scanner can image four channels simultaneously, due to its unique Variable Secondary Dichroic, all the while acquiring 3D images over multiple fields of view.

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HCBI: PALM Laser Microdissection Microscope
HCBI: PALM Laser Microdissection Microscope

Even the world’s greatest surgeon might struggle with dissecting single cells out of a slice of tissue. With the PALM system, laser microdissection, catapult and capture is as easy as drawing a region to dissect on the screen, and pressing start. Remove individual cells from your samples slices, or excise entire subregions for RT-PCR, DNA, or even mass spec analysis.

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HCBI: Zeiss Axio Scan.Z1
HCBI: Zeiss Axio Scan.Z1

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. Load your slides, let the software automatically locate your tissue sections, and off you go!

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HCBI: Training 3: Linda Liang
HCBI: Training 3: Linda Liang

Trains on all microscopes.

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HCBI: Training 1: Alex Lovely
HCBI: Training 1: Alex Lovely

Trains on all microscopes.

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HCBI: X-CLARITY Tissue Clearing System
HCBI: X-CLARITY Tissue Clearing System

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HCBI: Zeiss Axio Scan.Z1 #2
HCBI: Zeiss Axio Scan.Z1 #2

Have you ever looked at a giant pile of slides, and wondered how you'll find the time to image them all? The Axio Scan.Z1 is your answer! This high speed slide scanner has an astonishing 100-slide capacity, and can image samples stained with both colormetric and fluorescent dyes. Load your slides, let the software automatically locate your tissue sections, and off you go!

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HCBI: SmartClear II Pro
HCBI: SmartClear II Pro

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HCBI: Training 2: Heather Brown-Harding
HCBI: Training 2: Heather Brown-Harding

Trains on all microscopes.

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HCBI: Zeiss Lightsheet
HCBI: Zeiss Lightsheet

Long-term, high-speed 3D imaging with virtually no phototoxicity or bleaching may sound too good to be true, but the Lightsheet.Z1 delivers. This brand new microscope from ZEISS is the first of its kind to be installed in North America! Samples are immobilized in agarose or tubing, immersed in a buffer of your choosing, and can be freely rotated during imaging. 3D imaging that previously took hours on a point scanner can now be accomplished in mere minutes!

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HCBI: Zeiss LSM 980 + Airyscan
HCBI: Zeiss LSM 980 + Airyscan

The LSM 880 confocal microscope offers 34-channels of supreme sensitivity, owing to its GaAsP detectors with the added bonus of a super-resolution Airyscanner. This extraordinary point scanner can simultaneously image and spectrally separate up to 10 different dyes, create high resolution 3D images over large tiled areas, or image single molecules through fluorescence correlation techniques. The unparalleled sensitivity of this system also makes it the ideal live-cell imaging point scanner, allowing for high-speed confocal acquisition with brilliant signal to noise ratios. The Airy Scan array detector removes the requirement for a pinhole allowing ALL of the light collected from your sample to reach the detector. And additional deconvolution provides a 1.7x improvement over the theoretical diffraction limit.

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HCBI: Zeiss LSM 880 + FLIM
HCBI: Zeiss LSM 880 + FLIM

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OIC Training 1: Allston

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HCBI: MERSCOPE
HCBI: MERSCOPE

The MERSCOPE uses MERFISH technology to directly map and quantify the spatial distribution of hundreds to tens of thousands of RNA species in individual cells, without the need for downstream sequencing.

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OIC Axioscan

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OIC LSM 900 Bio

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OIC LSM 900 Materials

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HCBI: Zeiss LSM 900
HCBI: Zeiss LSM 900

The LSM 900 is optimal for confocal imaging with the four commonly used dyes. The entire system is fully motorized for autofocusing over large fields of view. It comes with an artificial intelligent (AI) Sample finder that enables the user to map their sample with flexibility to zone in on regions of interest. In addition to the confocal GaAsP detectors, the system has an Axiocam camera and an ESID for widefield fluorescence or transmitted light imaging.

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Holyoke: hcbiview01: Imaris only

High-end PC for graphics work running Volocity software from Improvision for 3D rendering and analysis of data including confocal data sets. In addition, this computer runs the free image-processing packages ImageJ and Fiji.

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Holyoke: hcbiview02: Arivis and ZEN

High-end PC for graphics work running ZEN Black Lightsheet addition. The computer features 15TB of data storage and is EXCLUSIVELY used for processing data sets acquired on the Lightsheet microscope.

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Holyoke: hcbiview03: Arivis and ZEN

High-end PC for graphics work running ZEN Black Lightsheet addition. The computer features 15TB of data storage and is EXCLUSIVELY used for processing data sets acquired on the Lightsheet microscope.

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Holyoke: hcbiview04: Arivis and ZEN

High-end PC for graphics work running ZEN Black Lightsheet addition. The computer features 15TB of data storage and is EXCLUSIVELY used for processing data sets acquired on the Lightsheet microscope.

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Holyoke: hcbiview05: Arivis, ZEN, Fiji, Cell Profiler, MATLAB

High-end PC for graphics work which includes a number of ZEN Black and ZEN Blue releases. The station is primarily used for processing 3DSIM and PALM/STORM data.

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Holyoke: hcbiview06: Arivis, ZEN, Fiji, Cell Profiler, MATLAB

High-end PC for graphics work which includes a number of ZEN Black and ZEN Blue releases. The station is primarily used for processing 3DSIM and PALM/STORM data.

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Holyoke: hcbiview07: ZEN, Fiji, Cell Profiler, MATLAB

High-end PC for graphics work which includes a number of ZEN Black and ZEN Blue releases. The station is primarily used for processing 3DSIM and PALM/STORM data.

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Holyoke: hcbiview08: ZEN, Fiji, Cell Profiler, Abberior

High-end PC for graphics work which includes a number of ZEN Black and ZEN Blue releases. The station is primarily used for processing 3DSIM and PALM/STORM data.

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Holyoke: hcbiview09: ZEN, Fiji, Cell Profiler, Bruker

High-end PC for graphics work which includes a number of ZEN Black and ZEN Blue releases. The station is primarily used for processing 3DSIM and PALM/STORM data.

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Holyoke: hcbiview10: ZEN, Fiji, Cell Profiler, Nikon Elements, Bruker

High-end PC for graphics work which includes a number of ZEN Black and ZEN Blue releases. The station is primarily used for processing 3DSIM and PALM/STORM data.

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HCBI: Nikon Spinning Disk
HCBI: Nikon Spinning Disk

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HCBI: LSM 900 - Live Cell
HCBI: LSM 900 - Live Cell

The LSM 900 is optimal for confocal imaging with the four commonly used dyes. The entire system is fully motorized for autofocusing over large fields of view. It comes with an artificial intelligent (AI) Sample finder that enables the user to map their sample with flexibility to zone in on regions of interest. In addition to the confocal GaAsP detectors, the system has an Axiocam camera and an ESID for widefield fluorescence or transmitted light imaging.

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HCBI: Abberior STED

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HCBI: Wohlwend High Pressure Freezer
HCBI: Wohlwend High Pressure Freezer

Wohlwend High Pressure Freezer

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HCBI: Yokogawa CV800 Spinning Disk
HCBI: Yokogawa CV800 Spinning Disk

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HCBI: Freeze Substitution #1
HCBI: Freeze Substitution #1

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HCBI: Freeze Substitution #2
HCBI: Freeze Substitution #2

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HCBI: Graphics Workstation 3: ZEN Black and Blue (all modules)

Please include the following acknowledgement in any publications utilizing HCBI resources: We thank the Harvard Center for Biological Imaging (RRID:SCR_018673) for infrastructure and support.

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Workstation 5: ZEN Blue (all modules)

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